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Analysis of three variable number terminal repeat loci is sufficient to characterize the deoxyribonucleic acid fingerprints of a panel of human tumor cell lines

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Summary

Using primers for the MCT118, YNZ22, and COL2A1 loci in polymerase chain reaction analysis we could distinguish among the ∼20 cell lines routinely maintained in our laboratory. We also demonstrated that the cell line NB-1691 (a neuroblastoma) and its xenograft had an identical number of repeats at two loci. Rh30 (a rhabdomyosarcoma) made resistant to rapamycin was identical to its parent line and to a subline that had reverted to sensitivity after it was cultured without rapamycin in the medium.

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Authors and Affiliations

  1. Department of Molecular Pharmacology, St. Jude Children's Research Hospital, 332 North Lauderdale Street, 38105, Memphis, Tennessee

    Allyson L. Anding, Tanika Reiss & Glen S. Germain

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  1. Allyson L. Anding
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  2. Tanika Reiss
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  3. Glen S. Germain
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Correspondence to Glen S. Germain.

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Anding, A.L., Reiss, T. & Germain, G.S. Analysis of three variable number terminal repeat loci is sufficient to characterize the deoxyribonucleic acid fingerprints of a panel of human tumor cell lines. In Vitro Cell.Dev.Biol.-Animal 39, 273–274 (2003). https://doi.org/10.1290/1543-706X(2003)039<0273:AOTVNT>2.0.CO;2

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  • DOI: https://doi.org/10.1290/1543-706X(2003)039<0273:AOTVNT>2.0.CO;2

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